Results: Representative iPSC-EP Vascular Network Generation and Analysis
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Conclusion
Transkript
Our protocol enables unique insights into the molecular and bulk tissue level mechanisms of vasculogenesis that may aid in the engineering functional vasculature for cardiovascular disease therapy in modeling. This protocol allows the creation of
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Endothelial progenitors derived from induced pluripotent stem cells (iPSC-EPs) have the potential to revolutionize cardiovascular disease treatments and to enable the creation of more faithful cardiovascular disease models. Herein, the encapsulation of iPSC-EPs in three-dimensional (3D) collagen microenvironments and a quantitative analysis of these cells’ vasculogenic potential are described.