Fluorescent Whole Mount In Situ Hybridization (WISH) Combined with Immunostaining
5:33
Live Imaging
8:25
Results: Fluorescent WISH and Live Imaging
9:17
Conclusion
Transkript
The protocol described here allow us to investigate the function of a chemokine and the behavior of macrophage in vivo. Most of existing experimental models of cell chemotaxis are based on in vitro cell experiments. But in vitro experiments someti
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To test the effect of a chemokine on macrophage recruitment in vivo, the whole mount in situ hybridization was used to detect the ectopic expression of the chemokine, and immunostaining was used to label macrophages. Live imaging was used for real-time observation of macrophage migration.