Fluorescent Whole Mount In Situ Hybridization (WISH) Combined with Immunostaining
5:33
Live Imaging
8:25
Results: Fluorescent WISH and Live Imaging
9:17
Conclusion
필기록
The protocol described here allow us to investigate the function of a chemokine and the behavior of macrophage in vivo. Most of existing experimental models of cell chemotaxis are based on in vitro cell experiments. But in vitro experiments someti
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
To test the effect of a chemokine on macrophage recruitment in vivo, the whole mount in situ hybridization was used to detect the ectopic expression of the chemokine, and immunostaining was used to label macrophages. Live imaging was used for real-time observation of macrophage migration.