Preparation of Stimulation Culture Medium for Cell Sheet Formation
5:44
Cell Sheet Formation by Culturing in Stimulation Medium
6:21
Preparation of 3D Tendon Stem Cell Construct
7:50
Assembly of the Uniaxial-Stretching Mechanical Stimulation Complex in Unique designed Bioreactor
9:25
Evaluation of the Mechanical Stimulated in vitro Tendon-like Tissue
10:10
Representative Results
11:07
Discussion
Transkript
Different mechanical loading parameters could induce tendon-derived STEM cells differentiated into different cells. We introduce detailed process of applying this three-dimensional uniaxial mechanical stimulation bioreactor system to induce tenoge
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A three-dimensional uniaxial mechanical stimulation bioreactor system is an ideal bioreactor for tenogenic-specific differentiation of tendon-derived stem cells and neo-tendon formation.