This method identifies viable ocular conjunctival microorganisms, which is challenging due to the eyes hostile environment. It can help answer whether an ocular microbiome exists and how bacterial presence changes in eye disease. In contrast to DN
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Presented here is a protocol for the isolation and amplification of aerobic and facultative anaerobic mouse conjunctival commensal bacteria using a unique eye swab and culture-based enrichment step with subsequent identification by microbiological based methods and MALDI-TOF mass spectrometry.