This protocol allowed to answer an important question in the cancer metastasis field. Including, how tumor cells colonize the lung? How tumor cells remain dormant?
And what induce them to exit dormancy and eventually form metastasis. The mai
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Here, we present a protocol for the surgical implantation of a permanently indwelling optical window for the murine thorax, which enables high-resolution, intravital imaging of the lung. The permanence of the window makes it well-suited to the study of dynamic cellular processes in the lung, especially those that are slowly evolving, such as metastatic progression of disseminated tumor cells.