Glycolysis Stress Test Using the Extracellular Flux Analyzer
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Results: Assessment of the Glycolytic Function and Mitochondrial Respiration in Mouse HSPCs
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Conclusion
Transkript
During hematopoiesis, hematopoietic stem cells undergo a metabolic switch from glycolysis to oxidative phosphorylation. This protocol can be used to assess glycolytic and mitochondrial functions of mouse hematopoietic stem and progenitor cells. Th
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The method presented here summarizes optimized protocols for assessing cellular bioenergetics in non-adherent mouse hematopoietic stem and primitive progenitor cells (HSPCs) using the extracellular flux analyzer to measure the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of HSPCs in real time.