Name: mammary epithelial transplant procedure
Uploaded: 2010-06-10T17:00:00
Description: Watch this Scientific Journal Video about Mammary Epithelial Transplant Procedure at JoVE.com

1。移植的上皮细胞的制备可以移植到新鲜或冷冻的捐助上皮组织清除的脂肪垫，但事先准备的冷冻捐助上皮在移植效率的最小下降（&gt; 85％的效率）更方便。编制冻结​​捐助上皮协议如下： 准备捐助移植上皮之前，准备冻结上皮和有关分装到冷冻管1毫升媒体。这可以保存在4 ° C，直到需要长达6个月。冻结媒体包含与HEPES和碳酸氢钠，10％胎牛血清（FBS...

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	<li>DeOme, K. B., Faulkin, L. J., Bern, H. A., Blair, P. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+mammary+tumors+from+hyperplastic+alveolar+nodules+transplanted+into+gland-free+mammary+fat+pads+of+female+C3H+mice.">Development of mammary tumors from hyperplastic alveolar nodules transplanted into gland-free mammary fat pads of female C3H mice.</a> Cancer Res. 19, 515-520 (1959).</li><li>Brill, B., Boecher, N., Groner, B., Shemanko, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+sparing+procedure+to+clear+the+mouse+mammary+fat+pad+of+epithelial+components+for+transplantation+analysis.">A sparing procedure to clear the mouse mammary fat pad of epithelial components for transplantation analysis.</a> Laboratory Animals. 42, 104-110 (2008).</li><li>Jerry, D. J., Kittrell, F. S., Kuperwasser, C., Laucirica, R., Dickinson, E. S., Bonilla, P. J., Butel, J. S., Medina, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+mammary-specific+model+demonstrates+the+role+of+the+p53+tumor+suppressor+gene+in+tumor+development.">A mammary-specific model demonstrates the role of the p53 tumor suppressor gene in tumor development.</a> Oncogene. 19, 1052-1058 (2000).</li><li>Shackleton, M., Vaillant, F., Simpson, K. J., Stingl, J., Smyth, G. K., Asselin-Labat, M. -. L., Wu, L., Lindman, G. J., Visvader, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generation+of+a+functional+mammary+gland+from+a+single+stem+cell.">Generation of a functional mammary gland from a single stem cell.</a> Nature. 439, 84-88 (2006).</li><li>Stingl, J., Eirew, P., Ricketson, I., Shackleton, M., Vaillant, F., Choi, D., Li, H. I., Eaves, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Purification+and+unique+properties+of+mammary+epithelial+stem+cells.">Purification and unique properties of mammary epithelial stem cells.</a> Nature. 439, 993-997 (2006).</li><li>Medina, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preneoplastic+Lesions+in+Murine+Mammary+Cancer.">Preneoplastic Lesions in Murine Mammary Cancer.</a> Cancer Res. 36, 2589-2595 (1976).</li><li>Wagner, K. -. U., Boulanger, C. A., Henry, S. g. a. g. i. a. s., Hennighausen, M., Smith, G. H. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+adjunct+mammary+epithelial+cell+population+in+parous+females:+its+role+in+functional+adaptation+and+tissue+renewal.">An adjunct mammary epithelial cell population in parous females: its role in functional adaptation and tissue renewal.</a> Development. 129, 1377-1386 (2002).</li><li>Dunphy, K. A., Blackburn, A. C., Haoheng, Y., O Connell, L. R., Jerry, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estrogen+and+progesterone+induce+persistent+increases+in+p53-dependent+apoptosis+and+suppress+mammary+tumors+in+BALB/c-Trp53+/-+mice.">Estrogen and progesterone induce persistent increases in p53-dependent apoptosis and suppress mammary tumors in BALB/c-Trp53+/- mice.</a> Breast Cancer Res. 10 (3), R43-R43 (2008).</li></ol>

在这份报告中，我们提出两个替代方法，结算准备乳腺上皮移植在小鼠乳腺脂肪垫。已在使用的标准程序，自1959年发展DeOme等。1虽然更具侵入性的，标准的方法是培训新手的首选方法，因为乳腺的结构和方向很容易观看。然而，饶程序可能会首选由更有经验的外科医生， 因为几个优点2。最重要的是，不遗余力程序是创伤小，需要更短的时间内，可以使用吸入麻醉的短暂时...

mammary epithelial transplant procedure

本文介绍和比较脂肪垫DeOme KB 等开发的清关程序 。1和备用程序开发布里尔 B 等 。2，乳腺上皮移植手术。乳腺移植手术被广泛应用于乳腺生物学家，因为它需要的事实，乳腺上皮细胞的显着发展，直到进入青春期后，不会发生的优势。在3周龄，乳腺上皮树的生长是局限于乳头附近，脂肪垫主要是缺乏对乳腺上皮细胞，但7周龄的乳腺导管上皮树延伸整个脂肪垫。因此，如果这种含有上皮细胞的脂肪垫一小部分，地区之间的乳头和淋巴结，是在3周拆除，内源性上皮永远不会填充乳腺脂肪垫，脂肪垫是描述为“清除“。这时，从其他来源的乳腺上皮可在清除脂肪垫，它有可能延长到了脂肪垫乳腺导管树木移植。利用这个程序已经在许多的实验模型，包括在转基因乳腺上皮组织的肿瘤表型无基因型的3对整个动物的混杂影响考试，在鉴别乳腺干细胞移植细胞有限稀释4,5的，确定增生性结节，如果进行乳腺肿瘤6，评估前激素对乳腺上皮细胞7,8的行为暴露的影响。 三个星期的旧主机小鼠麻醉，在手术阶段清理和克制。一个中期矢状切口，通过皮肤，但没有腹膜，从耻骨扩展到胸骨。斜削减从整个骨盆向每条腿中旬矢状切口，通过皮肤。皮肤被拉到远离腹膜暴露第四腹股沟乳腺。脂肪垫是通过消除脂肪垫组织前淋巴结清除。上皮碎片或上皮细胞移植到其余的清除脂肪垫和鼠标是封闭的。

Watch this Scientific Journal Video about Mammary Epithelial Transplant Procedure at JoVE.com

Mammary Epithelial Transplant Procedure

本文演示了由DeOme KB开发的程序<em&gt;等。</em&gt;（1959年）和备用程序开发布里尔乙<em&gt;等。</em&gt;（2008年）为结算第四腹股沟在乳腺片段，乳腺上皮细胞，或乳腺肿瘤细胞的移植准备柔毛小鼠乳腺脂肪垫。

乳腺上皮移植手术

This article describes and compares the fat pad clearance procedure developed by DeOme KB et al.1 and the sparing procedure developed by Brill B et al.2, ...

Research

JoVE Journal

Biology

Investigating the Immunological Mechanisms Underlying Organ Transplant Rejection

调查的基本免疫机制，器官移植排斥反应

科研

生物学

Murine Renal Transplantation Procedure

小鼠肾移植程序

Renal orthotopic transplantation in mice is a technically challenging procedure. Although the first kidney transplants in mice were performed by Russell ...

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

使用IVIS频谱的乳腺肿瘤在体内生物发光成像

4T1 mouse mammary tumor cells can be implanted sub-cutaneously in nu/nu mice to form palpable tumors in 15 to 20 days. This xenograft tumor model system ...

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation

小鼠乳腺上皮细胞形成过程中Lactogenic分化的微球体

A phenotypic measure commonly used to determine the degree of lactogenic differentiation in mouse mammary epithelial cell cultures is the formation of ...

Dendra2 Photoswitching through the Mammary Imaging Window

通过乳腺成像窗口Dendra2 Photoswitching

In the last decade, intravital microscopy of breast tumors in mice and rats at single-cell resolution1-4  has resulted in important insights into ...

Right Hemihepatectomy by Suprahilar Intrahepatic Transection of the Right Hemipedicle using a Vascular Stapler

右右Hemipedicle Suprahilar肝内血管吻合器横断Hemihepatectomy

Successful hepatic resection requires profound anatomical knowledge and delicate surgical technique. Hemihepatectomies are mostly performed after ...

Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

Tissue Determination Using the Animal Cap Transplant ACT Assay in Xenopus laevis

组织测定动物第移植（ACT）的含量非洲爪蟾</em

Many proteins play a dual role in embryonic development. Those that regulate cell fate determination in a specific tissue can also affect the development ...

Evaluation of Mammary Gland Development and Function in Mouse Models

乳腺的发育和功能在小鼠模型的评价

The human mammary gland is composed of 15-20 lobes that secrete milk into a branching duct system opening at the nipple. Those lobes are themselves ...

Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture

从三维混合细胞球体共培养的乳腺上皮细胞的隔离

While enormous efforts have gone into identifying signaling pathways and molecules involved in normal and malignant cell behaviors1-2, much of this work ...

Chromatin Isolation by RNA Purification (ChIRP)

Chromatin Isolation by RNA Purification ChIRP

RNA纯化的染色质隔离（CHIRP）

Long noncoding RNAs are key regulators of chromatin states for important biological processes such as dosage compensation, imprinting, and developmental ...