Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System

25.2K Views

•

08:21 min

•

September 16th, 2011

DOI :

10.3791/3473-v

September 16th, 2011

•

Gang Tian¹, Qing Lu², Li Zhang², Susanne E. Kohalmi¹, Yuhai Cui²

¹Department of Biology, University of Western Ontario, ²Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada

Summary

Chapters in this video

0:05

Title

1:16

Preparation of Agrobacterium culture for infiltration

4:06

Infiltration

6:03

Observation of the reconstituted YFP signal

7:40

Conclusion

Related Videos

07:57

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

16.4K Views

06:58

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

9.6K Views

10:39

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

19.5K Views

07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

10.7K Views

08:54

Bimolecular Fluorescence Complementation

27.8K Views

14:44

A Protocol for the Identification of Protein-protein Interactions Based on ¹⁵N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

20.4K Views

12:30

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

14.5K Views

11:11

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

18.3K Views

12:38

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

6.0K Views

11:10

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

20.8K Views