JoVE Journal

Neuroscience

A subscription to JoVE is required to view this content.

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in rodents.

Chapters in this video

0:05

Title

2:38

Dissection

4:51

Tissue Analysis: Total Protein and Final Normalization of Tyrosine Hydroxylase and Dopamine Active Transporter Results

8:12

Results: Tyrosine Hydroxylase Phosphorylation in Rat Substantia Nigra and Ventral Tegmental Samples

9:00

Conclusion

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More