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DOI :
10.3791/50405-v
•
12:37 min
August 22nd, 2013
Chapters
0:05
Title
2:40
Amplification of the Template
4:01
Vacuum Workstation Protocol
4:05
Immobilizing the Biotinylated PCR Products
5:06
Denaturation of DNA and Addition of Sequencing Primer
7:30
Annealing Sequencing Primer to DNA Strands
7:55
Pyromark Q24 Operation for Pyrosequencing
7:58
Preparing for a Run
8:58
Starting a Run
9:46
Completing a Run
10:03
Results: Pyrosequencing Identifies Bacteria and Detects Antibiotic Drug Resistance
11:35
Conclusion
焦磷酸测序法是一种通用的技术,可用于确定细菌种类,区分细菌的菌株,并检测抗微生物药物抗性的基因突变,有利于微生物的基因组测序。在这段视频中,微生物扩增子,扩增子的焦磷酸测序,DNA序列分析的程序将被证明。
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