JoVE Logo
Faculty Resource Center

Sign In

Laser Nanosurgery of Cerebellar Axons In Vivo

DOI :

10.3791/51371-v

July 28th, 2014

July 28th, 2014

8,052 Views

1European Laboratory for Non-Linear Spectroscopy, University of Florence, 2National Institute of Optics, National Research Council, 3Department of Physics and Astronomy, University of Florence, 4International Center for Computational Neurophotonics (ICON Foundation)

Two-photon imaging, coupled to laser nanodissection, are useful tools to study degenerative and regenerative processes in the central nervous system with subcellular resolution. This protocol shows how to label, image, and dissect single climbing fibers in the cerebellar cortex in vivo.

Tags

Keywords Laser Nanosurgery

-- Views

Related Videos

article

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

article

In vivo Laser Axotomy in C. elegans

article

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

article

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

article

Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy

article

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

article

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

article

In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model

article

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

article

Assessment of Long-term Depression Induction in Adult Cerebellar Slices

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved