We would like to thank Evan Rapoport and William Hulbert for writing the code for the original and updated Matrix programs.

NOTE: The following protocol follows the guidelines of Rutgers University human research ethics committee.
1. Stimuli
<ol>
	<li>Use sets of photographs that each belongs to the same category. Choose the number of stimuli that best fits the experimental question; much of the previous work with this procedure used 24 photographs per category;</li>
	<li>For the study of the detection of social threats, use schematic drawings or photographs of angry faces as the threatening stimuli, such as the adult angry faces found in the NimStim face set5, 7-9. Alternatively, use fearful faces5.</li>
	<li>For the study of the detection of social threats in children, use adult face stimuli such as the ones described above, or use photographs of child angry faces, such as those in the Child Affective Facial Expression set (CAFE)8, see note below. 
	Note: LoBue, V., &#38; Thrasher, C. The Child Affective Facial Expression (CAFE) Set: Validity and reliability from untrained adults. (2014).</li>
	<li>For the study of the detection of threatening animals such as snakes and spiders, use photographs of animals from nature books or websites3-4,7.</li>
	<li>Choose a category of neutral comparison stimuli that is well matched to the threatening category. If studying threatening (angry/fearful) faces, use neutral or happy faces as the comparison stimuli. If studying threatening animals (e.g., snakes/spiders), use a perceptually similar non-threatening animal (e.g., frogs/cockroaches) 3-4.</li>
	<li>Choose the distracter stimuli. Either interchange the targets and distracters (e.g., snake targets among frog distracters, and frog targets among snake distracters), or use a uniform set of distracters for the threatening and non-threatening target conditions (e.g., snake targets among flower distracters, and frog targets among flower distracters). 
	NOTE: See Discussion for issues with choosing appropriate distracters.</li>
	<li>When the stimuli are made up of photographs of faces, use an equal number of male and female faces, and vary the faces for race based on the availability of different races/ethnicities in each set.</li>
	<li>When the stimuli are made up of photographs of animals or plants, match the categories for color and brightness, or use black and white photographs3-4,7. </li>
</ol>
2. Equipment
<ol>
	<li>Obtain a computer with a touch-screen monitor for the task. Use a stand-alone touch-screen monitor that connects to standard VGA ports for any PC, or use a tablet PC that functions as an all-in-one computer and touch-screen.</li>
	<li>Choose the parameters of the study, including matrix size and number of trials. 
	NOTE: Previous work has used 9-picture (3 by 3) matrices, or 4-picture (2 by 2) matrices, and 24 trials, but other parameters may be used.</li>
	<li>Use either a customized program to present the matrices to participants, commercial presentation software such as EPrime, or access the Matrix program designed specifically for this methodology on the author&#8217;s website. 
	NOTE: The Matrix program allows for flexible study parameters. It gives researchers the option to choose matrix size, number of trials, and stimuli. It also randomly arranges the stimuli within each matrix, and presents them in a random order.</li>
	<li>Arrange the touch-screen monitor/computer at a desk or table with an outline of handprints located on the table in front of the monitor. Use the handprints as a starting point so that the participants&#8217; hands are in the same place for the start of each trial.</li>
</ol>
3. Child Procedure
<ol>
	<li>Ensure that vision impaired children are wearing their glasses or contact lenses throughout the procedure. Exclude vision impaired children who do not have a corrective device.</li>
	<li>Seat participants at arm&#8217;s length from the base of the touch-screen monitor before beginning the experiment.</li>
	<li>Instruct children to place their hands on the handprints. Do this between every trial so that the participants&#8217; hands are in the same place at the beginning of every trial.</li>
	<li>Stand alongside the monitor to instruct the participant throughout the procedure.</li>
	<li>First explain the task to the child: &#8220;Are you ready to play a computer game with me? This is a special computer that you can touch! I&#8217;m going to show you a bunch of pictures on the screen and ask you to touch some of them. Are you ready?&#8221;</li>
	<li>Next, teach the child participants how to use the touch-screen by giving them several practice trials. On the first practice trial, present participants with a single photograph from the target category, and ask them to touch it on the screen. Use the following language: &#8220;This is a (target). Can you touch the (target) on the screen?&#8221;</li>
	<li>On the second practice trial, present participants with a single photograph from the distracter category, and ask them to touch it on the screen. Use the following language: &#8220;This is a (distracter). Can you touch the (distracter) on the screen?&#8221;</li>
	<li>On the next three practice trials, present participants with full nine-picture matrices with one target among eight distracters. When the first nine-picture practice matrix appears on the screen, give the following instructions: &#8220;When you see the pictures come up on the screen, it&#8217;s your job to find the (target) and touch it as fast as you can. Can you do that? Do you think you can find the (targets) really fast?&#8221; 
	NOTE: The procedure can be modified for matrices of other sizes, such as 2 &#215; 2, 1 &#215; 1, etc.</li>
	<li>Between each full-matrix trial, design the stimulus presentation program so that a smiley face icon appears. Explain to the child: &#8220;It&#8217;s your job to touch the (targets), and it&#8217;s my job to touch the smiley face.&#8221; Reinforce these directions if the child attempts to touch the smiley face between subsequent trials.</li>
	<li>Use the smiley face between each trial to ensure that the child&#8217;s full attention is on the screen before the onset of the next trial. When the child&#8217;s hands are on the handprints and he/she is looking at the screen, press the smiley face icon to continue. Do this between every trial.</li>
	<li>Touch the smiley face and continue to the second and third practice trials. If the child does not touch the target on the screen, reiterate the instructions: &#8220;Remember, your job is to find the (target) as fast as you can and touch it on the screen!&#8221;</li>
	<li>Next, present participants with the test trials.</li>
	<li>Use a stimulus presentation program that automatically records latency to touch the screen from the onset of each matrix. Present matrices on the screen until the participants touch the target. Do not record latency when the smiley face icon is displayed; use this icon to redirect the child&#8217;s attention to the screen, and to reiterate instructions if necessary.</li>
	<li>Identify errors from your latency data. Errors are trials in which participants select one of the distracter stimuli instead of the target. Custom stimulus presentation software should be written so that errors are identified and marked in the output.</li>
	<li>Calculate average latency to detect the target stimuli for each participant after eliminating errors. Use these data for the statistical analyses.</li>
</ol>
4. Adult Procedure
<ol>
	<li>Ensure that vision impaired adults are wearing their glasses or contact lenses throughout the procedure. Exclude vision impaired adults who do not have a corrective device.</li>
	<li>Seat participants at arm&#8217;s length from the base of the touch-screen monitor.</li>
	<li>Instruct the participant to place his/her hands on the handprints to ensure that the participants&#8217; hands are in the same place at the onset of every trial.</li>
	<li>Stand alongside the monitor to instruct the participant throughout the procedure.</li>
	<li>In order to teach participants how to use the touch-screen, give them several practice trials. In the first two practice trials, ask participants to touch a single picture from the target category on the screen, followed by a single picture from the distracter category.</li>
	<li>On the next three practice trials, present participants with full nine-picture matrices with one target amid eight distracters.</li>
	<li>Instruct participants to find the targets and touch them on the screen as quickly as possible. Then return his/her hands to the handprints. 
	NOTE: The smiley face between each trial is not necessary for adult participants; you can choose whether to use it or eliminate it.</li>
	<li>If using the smiley faces, instruct participants to touch the smiley face to move on to the next trial.</li>
	<li>Following practice trials, present participants the test trials, each containing one target and eight distracters.</li>
	<li>Use a stimulus presentation program that automatically records latency to touch the screen from the onset of each matrix.</li>
	<li>Identify errors from your latency data, as specified in step 3.13.</li>
	<li>Calculate average latency to detect the target stimuli for each participant after eliminating errors. Use these data for the statistical analyses.</li>
</ol>

<ol>
	<li>Flykt, A., Esteves, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Emotion+drives+attention:+Detecting+the+snake+in+the+grass.">Emotion drives attention: Detecting the snake in the grass.</a> J. Exp. Psychol. Gen. 130 (3), 466-478 (2001).</li><li>Lundqvist, D., Esteves, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+face+in+the+crowd+revisited:+A+threat+advantage+with+schematic+stimuli.">The face in the crowd revisited: A threat advantage with schematic stimuli.</a> J. Pers. Soc. Psychol. 80 (3), 381-396 (2001).</li><li>LoBue, V., DeLoache, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detecting+the+snake+in+the+grass:+Attention+to+fear-relevant+stimuli+by+adults+and+young+children.">Detecting the snake in the grass: Attention to fear-relevant stimuli by adults and young children.</a> Psychol. Sci. 19 (3), 284-289 (2008).</li><li>LoBue, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=And+along+came+a+spider:+Superior+detection+of+spiders+in+children+and+adults.">And along came a spider: Superior detection of spiders in children and adults.</a> J. Exp. Child. Psychol. 107 (1), 59-66 (2010).</li><li>LoBue, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=More+than+just+a+face+in+the+crowd:+Detection+of+emotional+facial+expressions+in+young+children+and+adults.">More than just a face in the crowd: Detection of emotional facial expressions in young children and adults.</a> Developmental Sci. 12 (2), 305-313 (2009).</li><li>Shibasaki, M., Kawai, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+detection+of+snakes+by+Japanese+Monkeys+(Macaca+fuscata):+An+evolutionarily+predisposed+visual+system.">Rapid detection of snakes by Japanese Monkeys (Macaca fuscata): An evolutionarily predisposed visual system.</a> J. Comp. Psychol. 123 (2), 131-135 (2009).</li><li>LoBue, V., DeLoache, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=What+so+special+about+slithering+serpents?+Children+and+adults+rapidly+detect+snakes+based+on+their+simple+features.">What so special about slithering serpents? Children and adults rapidly detect snakes based on their simple features.</a> Vis. Cogn. 19 (1), 129-143 (2011).</li><li>LoBue, V., Matthews, K., Harvey, T., Thrasher, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pick+on+someone+your+own+size:+The+detection+of+threatening+facial+expressions+posed+by+both+child+and+adult+models.">Pick on someone your own size: The detection of threatening facial expressions posed by both child and adult models.</a> J. Exp. Child. Psychol. 118, 134-142 (2014).</li><li>Tottenham, N., Tanaka, J. W., Leon, A. C., McCarry, T., Nurse, M., Hare, T. A., Marcus, D. J., Westerlund, A., Casey, B. J., Nelson, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+NimStim+set+of+facial+expressions:+Judgments+from+untrained+research+participants.">The NimStim set of facial expressions: Judgments from untrained research participants.</a> Psychiat. Res. 168 (3), 242-249 (2009).</li><li>LoBue, V., P&#233;rez-Edgar, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sensitivity+to+social+and+non-social+threats+in+temperamentally+shy+children+at-risk+for+anxiety.">Sensitivity to social and non-social threats in temperamentally shy children at-risk for anxiety.</a> Developmental Sci. 17 (2), 239-247 (2014).</li><li>LoBue, V., Matthews, K., Harvey, T., Stark, S. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=What+accounts+for+the+rapid+detection+of+threat?+Evidence+for+an+advantage+in+perceptual+and+behavioral+responding+from+eye+movements.">What accounts for the rapid detection of threat? Evidence for an advantage in perceptual and behavioral responding from eye movements.</a> Emotion. 14, 816-823 (2014).</li><li>Baayen, R. H., Davidson, D. J., Bates, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mixed-effects+modeling+with+crossed+random+effects+for+subjects+and+items.">Mixed-effects modeling with crossed random effects for subjects and items.</a> J Mem Lang. 59 (4), 390-412 (2008).</li><li>Bagiella, E., Sloan, R. P., Heitjan, D. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mixed-effects+models+in+psychophysiology.">Mixed-effects models in psychophysiology.</a> Psychophysiology. 37 (1), 13-20 (2008).</li><li>Gueorguieva, R., Krystal, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Move+over+ANOVA:+Progress+in+analyzing+repeated-measures+data+and+its+reflection+in+papers+published+in+the+Archives+of+General+Psychiatry.">Move over ANOVA: Progress in analyzing repeated-measures data and its reflection in papers published in the Archives of General Psychiatry.</a> Arch. Gen. Psychiat. 61 (3), 310-317 (2004).</li><li>LoBue, V., Matthews, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+snake+in+the+grass+revisited:+An+experimental+comparison+of+threat+detection+paradigms.">The snake in the grass revisited: An experimental comparison of threat detection paradigms.</a> Cognition Emotion. 28 (1), 22-35 (2014).</li><li>LoBue, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Deconstructing+the+snake:+The+relative+roles+of+perception,+cognition,+and+emotion+on+threat+detection.">Deconstructing the snake: The relative roles of perception, cognition, and emotion on threat detection.</a> Emotion. 14, 701-711 (2014).</li><li>Lipp, O. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Of+snakes+and+flowers:+Does+preferential+detection+of+pictures+of+fear-relevant+animals+in+visual+search+reflect+on+fear-relevance.">Of snakes and flowers: Does preferential detection of pictures of fear-relevant animals in visual search reflect on fear-relevance.</a> Emotion. 6 (2), 296-308 (2006).</li><li>Lipp, O. V., Derakshan, N., Waters, A. M., Logies, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Snakes+and+cats+in+the+flower+bed:+Fast+detection+is+not+specific+to+pictures+of+fear-relevant+animals.">Snakes and cats in the flower bed: Fast detection is not specific to pictures of fear-relevant animals.</a> Emotion. 4 (3), 233-250 (2004).</li><li>Tipples, J., Young, A. W., Quinlan, P., Broks, P., Ellis, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Searching+for+threat.">Searching for threat.</a> Q. J. Exp. Psychol. 55 (3), 1007-1026 (2002).</li><li>Frischen, A., Eastwood, J. D., Smilek, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visual+search+for+faces+with+emotional+expressions.">Visual search for faces with emotional expressions.</a> Psychol. Bull. 134 (5), 662-676 (2008).</li><li>Byrne, A., Eysenck, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trait+anxiety,+anxious+mood,+and+threat+detection.">Trait anxiety, anxious mood, and threat detection.</a> Cognition Emotion. 9 (6), 549-562 (1995).</li><li>Fenske, M. J., Eastwood, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modulation+of+focused+attention+by+faces+expressing+emotion:+Evidence+from+flanker+tasks.">Modulation of focused attention by faces expressing emotion: Evidence from flanker tasks.</a> Emotion. 3 (4), 327-343 (2003).</li><li>Fox, E., Russo, R., Dutton, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Attentional+bias+for+threat:+Evidence+for+delayed+disengagement+from+emotional+faces.">Attentional bias for threat: Evidence for delayed disengagement from emotional faces.</a> Cognition Emotion. 16 (3), 355-379 (2002).</li><li>Gilboa-Schechtman, E., Foa, E. B., Amir, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Attentional+biases+for+facial+expressions+in+social+phobia:+The+face-in-the-crowd+paradigm.">Attentional biases for facial expressions in social phobia: The face-in-the-crowd paradigm.</a> Cognition Emotion. 13 (3), 305-318 (1999).</li><li>Horstmann, G., Scharlau, I., Ansorge, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=More+efficient+rejection+of+happy+than+of+angry+face+distractors+in+visual+search.">More efficient rejection of happy than of angry face distractors in visual search.</a> Psychon. B. Rev. 13 (6), 1067-1073 (2006).</li><li>Lipp, O. V., Waters, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=When+danger+lurks+in+the+background:+Attentional+capture+by+animal+fear-relevant+distractors+is+specific+and+selectively+enhanced+by+animal+fear.">When danger lurks in the background: Attentional capture by animal fear-relevant distractors is specific and selectively enhanced by animal fear.</a> Emotion. 7 (1), 192-200 (2007).</li><li>Cacioppo, J. T., Priester, J. R., Berntson, G. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rudimentary+determinants+of+attitudes:+II.+Arm+flexion+and+extension+have+differential+effects+on+attitudes.">Rudimentary determinants of attitudes: II. Arm flexion and extension have differential effects on attitudes.</a> J. Pers. Soc. Psychol. 65 (1), 5-17 (1993).</li><li>Reynolds, M. G., Eastwood, J. D., Partanen, M., Frischen, A., Smilek, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monitoring+eye+movements+while+searching+for+affective+faces.">Monitoring eye movements while searching for affective faces.</a> Vis. Cogn. 17 (3), 318-333 (2009).</li><li>Calvo, M. G., Avero, P., Lundqvist, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Facilitated+detection+of+angry+faces:+Initial+orienting+and+processing+efficiency.">Facilitated detection of angry faces: Initial orienting and processing efficiency.</a> Cognition Emotion. 20 (6), 785-811 (2006).</li><li>Reinholdt-Dunne, M. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anxiety+and+selective+attention+to+angry+faces:+An+antisaccade+study.">Anxiety and selective attention to angry faces: An antisaccade study.</a> J. Cogn. Psychol. 24 (1), 54-65 (2012).</li><li>Rinck, M., Reinecke, A., Ellwart, T., Heuer, K., Becker, E. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Speeded+detection+and+increased+distraction+in+fear+of+spiders:+Evidence+from+eye+movements.">Speeded detection and increased distraction in fear of spiders: Evidence from eye movements.</a> J. Abnorm. Psychol. 114 (2), 235-248 (2005).</li><li>Flykt, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preparedness+for+action:+Responding+to+the+snake+in+the+grass.">Preparedness for action: Responding to the snake in the grass.</a> J. Abnorm. Psychol. 119 (1), 29-43 (2006).</li><li>Flykt, A., Caldera, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tracking+fear+in+snake+and+spider+fearful+participants+during+visual+search:+A+multi-response+domain+study.">Tracking fear in snake and spider fearful participants during visual search: A multi-response domain study.</a> Cognition Emotion. 20 (8), 1075-1091 (2006).</li></ol>

The authors have nothing to disclose.

Here a detailed protocol for the child-friendly touch-screen detection paradigm is presented, and previous results using the procedure with children and adults are discussed. There are some additional factors that researchers should consider when using the paradigm. First, researchers should think carefully about the experimental set-up, as labeling the targets, the participant&#8217;s emotional state, and the participant&#8217;s emotional traits (e.g., phobias, anxiety) have all been shown to affect the results1,11,15-16. Further, researchers should take caution in choosing appropriate target stimuli. In much of the research on threat detection with adults, detection of snakes and spiders was compared to detection of flowers and mushrooms1. However, since flowers and mushrooms are not animals, the advantage for snakes and spiders reported in these studies might reflect an advantage for animals in general and not threatening animals per se. A handful of studies have shown that animals (regardless of threat-relevance) are detected more quickly than plants17-19; comparing snakes and spiders to other animals would rectify this potential issue3-4. Similar attention should be paid to choosing appropriate distracter stimuli for visual detection studies as threatening distracters have been shown to slow down participants when they are detecting non-threatening targets20-26. Using uniform distracters might help ensure that any differences found in detection can be attributed to the targets7. Finally, when choosing both target and distracter stimuli, attention should be paid to perceptual heterogeneity across the stimuli. In other words, photographs should be matched for color, brightness, luminance, etc., as visual search paradigms are particularly sensitive to low-level perceptual differences of the stimuli.
A potential criticism of the touch-screen paradigm is that it requires participants to make physical contact with the target stimuli by touching them on the screen. One could argue that requiring participants to make physical contact with photographs of threatening stimuli might slow responding instead of facilitating it. However, extensive work using the touch-screen paradigm has shown consistently that threatening stimuli are detected (and touched on the screen) more quickly than a variety of non-threatening stimuli, even if the participants are phobic or afraid of the threatening targets1. Further, several studies have suggested that the pressing motion required for the touch-screen detection paradigm is indeed in line with avoidance responding. More specifically, Cacioppo and colleagues have suggested that the act of pulling towards oneself is generally associated with approaching positive stimuli, while the act of pushing produces feedback in the body that is akin to avoiding negative stimuli. For example, participants who were asked to rate neutral stimuli during an arm flexion task preferred the stimuli more than participants who rated them during an arm extension task27. Thus, although the touch-screen procedure requires participants to make physical contact with threatening stimuli, there is no evidence to suggest that making physical contact with these threats slows responding.
One final note is that the touch-screen procedure can now be used in combination with eye tracking technology, which can allow for the potential to uncover the mechanisms that drive rapid threat detection. Some researchers, for example, have suggested that the advantage for threat in visual search paradigms is driven by rapid first fixations to threatening stimuli28. Others have reported that these results are driven by the fact that participants make fewer fixations before detecting threatening than non-threatening stimuli29. In contrast, other researchers have shown that the advantage for threat in anxious or phobic participants is driven by difficulty disengaging from the object of the participants&#8217; fear30-31. Finally, there are others who have suggested that the advantage for threat in detection paradigms is due to faster behavioral responding (pressing a button or touching a screen) after threatening targets are first fixated. In other words, threatening stimuli might evoke faster action, and not necessarily faster detection32-33. Using the touch-screen paradigm in combination with eye-tracking technology can help to clarify this important (and still controversial) issue.
In conclusion, the child-friendly touch-screen paradigm produces results similar to those produced with traditional adult-focused visual detection paradigms. Future research using this paradigm might not only help elucidate the kinds of stimuli that are detected particularly quickly, but it may also help uncover how humans acquire these biases for threat in visual attention.

For decades researchers have been interested in humans&#8217; detection of various types of threatening stimuli. In the standard adult detection paradigm used in previous research, participants are generally presented with photographs arranged in 3 &#215; 3 matrices or 2 &#215; 2 matrices. The matrices are comprised of photos from a single stimulus category, or they contain one discrepant image from a second stimulus category. Adult participants are asked to press one button if all of the photos are from the same category, and a second button if there is a discrepant image present. Adults generally detect threatening stimuli including snakes, spiders, and angry faces more quickly than neutral stimuli including flowers, mushrooms, and happy or neutral faces1,2.
Traditionally, the focus of most threat detection research has been on adult participants. In order to examine how attentional biases for threat develop, LoBue and DeLoache (2008) modified the standard adult visual detection paradigm so that it could be used with children as well3. They presented participants with 3 &#215; 3 matrices of images on a touch-screen monitor, each matrix containing a single target among eight distracters. They told participants to find the target as quickly as possible and touch it on the screen. Various studies using the modified touch-screen paradigm have shown results parallel to those reported in previous research with the standard button-press procedure described above: Preschool children (ranging in age from 3 to 5) and adults detect images of snakes faster than images of flowers, frogs, and caterpillars; they detect spiders faster than mushrooms and cockroaches; and they detect angry and fearful facial expressions faster than happy, neutral, and sad faces3-5.
There are a few important differences between the standard button-press procedure and the new touch-screen procedure that make the touch-screen paradigm easier and child-friendly. In the classic adult procedure, participants are presented with two types of matrices&#8212;some that are made up of photographs from a single category, and others that contain an image from a discrepant category. In this paradigm, participants&#8217; task is to press one key if they see a discrepant image, and a second key if all of the images in the matrix belong to the same category. In contrast, participants using the touch-screen procedure know that there will be a target in every matrix, and their task is to simply touch it. This makes the touch-screen task easier: Instead of having to detect whether a discrepant image is present and remember to press a specific button on a keyboard, participants in the touch-screen paradigm know that a target is present in every matrix, and their only task is to find it and touch it directly on the screen5. Further, the touch-screen procedure can be thought of as a forced choice task as opposed to a yes/no task like the standard button-press procedure; using a forced choice task eliminates any potential response bias. The touch-screen methodology can be used with children as young as three, with older children, and with adults. In fact, researchers have even used the touch-screen paradigm to examine threat detection in monkeys, reporting that they too detect a single snake among eight flowers more quickly than a single flower among eight snakes6.
Here we present a detailed protocol for the child-friendly touch-screen detection paradigm, describing the relevant materials, equipment, procedure, and analyses required to use this procedure with both child and adult participants. We describe previous results using the standard adult button-press procedure and the modified touch-screen procedure within the same participants and discuss similarities and differences between the results for each paradigm. Finally, we discuss practical considerations for using the touch-screen procedure in future research on the study of threat detection.

measuring attentional biases for threat in children and adults

Investigators have long been interested in the human propensity for the rapid detection of threatening stimuli. However, until recently, research in this domain has focused almost exclusively on adult participants, completely ignoring the topic of threat detection over the course of development. One of the biggest reasons for the lack of developmental work in this area is likely the absence of a reliable paradigm that can measure perceptual biases for threat in children. To address this issue, we recently designed a modified visual search paradigm similar to the standard adult paradigm that is appropriate for studying threat detection in preschool-aged participants. Here we describe this new procedure. In the general paradigm, we present participants with matrices of color photographs, and ask them to find and touch a target on the screen. Latency to touch the target is recorded. Using a touch-screen monitor makes the procedure simple and easy, allowing us to collect data in participants ranging from 3 years of age to adults. Thus far, the paradigm has consistently shown that both adults and children detect threatening stimuli (e.g., snakes, spiders, angry/fearful faces) more quickly than neutral stimuli (e.g., flowers, mushrooms, happy/neutral faces). Altogether, this procedure provides an important new tool for researchers interested in studying the development of attentional biases for threat.

Statistical Analyses
There are several possible statistical analyses that can be done with data produced by the touch-screen methodology. Use SPSS or other statistical software to analyze the data. The original studies using the touch-screen detection task utilized between-subjects designs where each participant was randomly assigned to one experimental condition3,5. If this is the case, investigators should calculate the average latency to detect the target stimuli on all usable trials (as directed in the Protocol). This produces a single data point for each participant. The data can then be entered as the dependent variable in a standard ANOVA with target category as the between-subjects factor.
Alternatively, researchers may choose to use a within-subjects design with participants receiving all of the experimental conditions. In this case, researchers should be careful to counterbalance/randomize the order of tasks, as participants tend to get faster over repeated trials. Researchers can use the same statistical methods described above for the between-subjects design using a repeated measures ANOVA. Alternatively, an advantage of a within-subjects design is that researchers can calculate a bias score, which is generally a difference score that represents a bias for certain kinds of stimuli. For example, a bias score for threatening faces can be calculated by subtracting the average latency to detect angry faces from average latency to detect happy faces10. In this case, positive scores indicate a bias for threats, and negative scores indicate a bias for non-threats.
In some cases, researchers might choose to use within-subjects designs where participants complete several experimental conditions in a single testing session. In these cases, researchers might choose to use Mixed Effects ANOVAs to analyze trial-level data instead of analyzing a single averaged data point for each participant. By using every data point instead of a single mean, Mixed Models take into account individual differences in a participants&#8217; behavior over the course of many trials, reducing the potential for error12-14.
Finally, it is worth noting that head-mounted or desk-mounted eye-tracking technology can be used in combination with the touch-screen visual detection paradigm to capture exact fixations as participants search for target stimuli. Eye-tracking produces more than just latency to touch the screen&#8212;it also produces data on latency to first fixate the target, total fixations and fixation time to each distracter before first fixating the target, and latency from the first fixation to making a behavioral response11. By differentiating between these measures, researchers can disambiguate the potential mechanisms that drive rapid detection. For example, a perceptual advantage for target stimuli can be examined by analyzing latency to first fixate target stimuli. If there is a perceptual advantage for some stimuli over others, latency to first fixate those targets should be faster than for other targets. Automaticity of search, or &#8220;pop out,&#8221; can also be measured using an eye-tracker by examining the number of distracters each participant fixates before they reach the target. If search occurs automatically for certain target stimuli, participants should scan fewer distracters before reaching those targets. An eye-tracker can also be used to examine efficiency of behavioral responding, measuring latency to touch the screen from the time the participant first fixates the target. If there is an advantage in behavioral responding for certain target stimuli, participants should be faster to make a behavioral response (e.g., touch a target on the screen) after first fixating those targets. Mixed Models can be used to analyze eye-tracking data so that each fixation can be used in the analyses.
Patterns of Detection in Preschool Children and Adults
Previous research using the touch-screen detection paradigm with both child and adult participants has consistently shown that participants of all ages detect threatening stimuli more quickly than non-threatening stimuli. In the original paper using the procedure, the authors examined detection of snakes versus various non-threatening stimuli (flowers, frogs, and caterpillars respectively). In the procedure for Experiment 1, participants either detected a single snake among 8 flowers or a single flower among 8 snakes on each subsequent trial. Participants detected snakes more quickly than flowers, and adults detected all of the stimuli more quickly than children. A second experiment compared snakes to an animal that closely resembles snakes&#8212;frogs. Again, participants detected the snakes significantly faster than the frogs, and the adults detected all targets more quickly than children. Finally, a third experiment compared detection of snakes to another animal that is shaped like a snake&#8212;caterpillars. Again, both age groups detected snakes more quickly than caterpillars, but the effect was only significant for children3 (Figure 1).
<img alt="Figure 1" src="/files/ftp_upload/52190/52190fig1highres.jpg" /> 
Figure 1 represents the data collected for 3-year-olds and adults in Experiments 1&#8211;3, and has been modified from LoBue &#38; DeLoache (2008)3. In all three experiments, 3-year-olds detected threatening stimuli (snakes) significantly faster than various non-threatening stimuli (flowers, frogs, and caterpillars respectively). Adults showed the same pattern, but the results were only significant for Experiments 1 and 2 (flowers, frogs). This figure has been modified from LoBue &#38; DeLoache (2008)3. <a href="https://www.jove.com/files/ftp_upload/52190/52190fig1highres.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
Together, these results show that the touch-screen paradigm demonstrates an advantage for threatening over non-threatening stimuli&#8212;the same advantage reported in previous research. Further, the touch-screen paradigm produces the same patterns of responding across multiple age groups including adults and preschool-aged children3-5.
Comparison Across Paradigms
How do the results produced by the touch-screen procedure compare to results produced by the classic adult button-press detection paradigm? One study recently sought to replicate the button-press1 and touchscreen3 methodologies within subjects to compare patterns of responding across these two paradigms15. In the study, a group of adults performed both the button-press detection and touch-screen detection tasks exactly, and the results were compared. As expected, in both paradigms, participants detected threatening targets (snakes, spiders) more quickly and accurately (i.e., they made fewer errors) than non-threatening targets (flowers, mushrooms), consistent with previous work using both paradigms. These results show that the touch-screen paradigm indeed produces the same pattern of results as the classic button-press procedure,3-4,15, suggesting that minor differences between the paradigms (stimuli, number of trials, etc.) do not change the overall pattern of results with respect to the detection of threatening versus non-threatening stimuli.
Despite these similarities, there was also one important difference in the results worth noting. In the button-press procedure, increasing the matrix size from 4 to 9 photographs slowed detection of non-threatening targets, whereas detection of threatening targets was equally fast regardless of set size. No such interaction was found for the touch-screen paradigm, and detection of threatening and non-threatening targets was slower when increasing the matrix size from 4 to 9 photographs. Further, there was little relationship between responding in one task and responding in the other according to a correlational analysis. Thus, researchers should keep in mind that although the overall pattern of results&#8212;faster detection of threatening versus non-threatening stimuli&#8212;was the same between paradigms, it is still unclear whether the procedures are measuring the same underlying process15 (Figure 2).
<img alt="Figure 2" src="/files/ftp_upload/52190/52190fig2highres.jpg" /> 
Figure 2 represents the data collected from adults (and has been modified from the figure) in LoBue &#38; Matthews (2014)15. It presents average latencies to detect target stimuli in the button-press procedure (Experiment 1), and the touch-screen procedure (Experiment 3). Both procedures produced an advantage for threatening stimuli&#8212;snakes and spiders were detected more quickly than flowers and mushrooms. However, there was only a target by set size interaction for the button-press procedure, indicating that detection of threatening stimuli was not affected by number of distracters in each matrix, whereas detection of non-threatening stimuli was faster in 2 &#215; 2 than in 3 &#215; 3 matrices. Such an interaction was not found for the touch-screen procedure, and both types of stimuli were equally affected by increasing the matrix size from 4 to 9 photographs. This figure has been modified from LoBue &#38; Matthews (2014)15. <a href="https://www.jove.com/files/ftp_upload/52190/52190fig2highres.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

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Measuring Attentional Biases for Threat in Children and Adults

Here we describe a touch-screen visual search paradigm that can be used to study threat detection across the lifespan. The paradigm has already been used in various studies demonstrating that both children and adults detect threatening stimuli like snakes, spiders, and angry faces faster than non-threatening stimuli.

Investigators have long been interested in the human propensity for the rapid detection of threatening stimuli. However, until recently, research in this ...

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15.2K Views.  Rutgers University. Here we describe a touch-screen visual search paradigm that can be used to study threat detection across the lifespan. The paradigm has already been used in various studies demonstrating that both children and adults detect threatening stimuli like snakes, spiders, and angry faces faster than non-threatening stimuli.

Video: Measuring Attentional Biases for Threat in Children and Adults

Measurement of Fronto-limbic Activity Using an Emotional Oddball Task in Children with Familial High Risk for Schizophrenia

Adolescence is a critical developmental period where the early symptoms of schizophrenia frequently emerge. First-degree relatives of people with schizophrenia who are at familial high risk (FHR) may show similar cognitive and emotional changes. However, the neurological changes underlying the emergence of these symptoms remain unclear. This study sought to identify differences in frontal, striatal, and limbic regions in children and adolescents with FHR using functional magnetic resonance imaging. Groups of 21 children and adolescents at FHR and 21 healthy controls completed an emotional oddball task that relied on selective attention and the suppression of task-irrelevant emotional information. The standard oddball task was modified to include aversive and neutral distractors in order to examine potential group differences in both emotional and executive processing. This task was designed specifically to allow for children and adolescents to complete by keeping the difficulty and emotional image content age-appropriate. Furthermore, we demonstrate a technique for suitable fMRI registration for children and adolescent participants. This paradigm may also be applied in future studies to measure changes in neural activity in other populations with hypothesized developmental changes in executive and emotional processing.

This paper describes how to use the emotional oddball task and fMRI to measure brain activation in children and adolescents at familial high risk for schizophrenia (FHR).&#160;FMRI was used to measure differences in fronto-striato-limbic regions during an emotional oddball task. Children with FHR exhibited abnormal functional activation during adolescence.

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Cortical Source Analysis of High-Density EEG Recordings in Children

EEG is traditionally described as a neuroimaging technique with high temporal and low spatial resolution. Recent advances in biophysical modelling and signal processing make it possible to exploit information from other imaging modalities like structural MRI that provide high spatial resolution to overcome this constraint1. This is especially useful for investigations that require high resolution in the temporal as well as spatial domain. In addition, due to the easy application and low cost of EEG recordings, EEG is often the method of choice when working with populations, such as young children, that do not tolerate functional MRI scans well. However, in order to investigate which neural substrates are involved, anatomical information from structural MRI is still needed. Most EEG analysis packages work with standard head models that are based on adult anatomy. The accuracy of these models when used for children is limited2, because the composition and spatial configuration of head tissues changes dramatically over development3.&#160;
In the present paper, we provide an overview of our recent work in utilizing head models based on individual structural MRI scans or age specific head models to reconstruct the cortical generators of high density EEG. This article describes how&#160;EEG recordings are acquired, processed, and analyzed with pediatric populations at the London Baby Lab, including laboratory setup, task design, EEG preprocessing, MRI processing, and EEG channel level and source analysis.&#160;

In recent years, there has been increasing interest in estimating the cortical sources of scalp measured electrical activity for cognitive neuroscience experiments. This article describes how high density EEG is acquired and how recordings are processed for cortical source estimation in children from the age of 2 years at the London Baby Lab.

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Using the Threat Probability Task to Assess Anxiety and Fear During Uncertain and Certain Threat

Fear of certain threat and anxiety about uncertain threat are distinct emotions with unique behavioral, cognitive-attentional, and neuroanatomical components. Both anxiety and fear can be studied in the laboratory by measuring the potentiation of the startle reflex. The startle reflex is a defensive reflex that is potentiated when an organism is threatened and the need for defense is high. The startle reflex is assessed via electromyography (EMG) in the orbicularis oculi muscle elicited by brief, intense, bursts of acoustic white noise (i.e., &#8220;startle probes&#8221;). Startle potentiation is calculated as the increase in startle response magnitude during presentation of sets of visual threat cues that signal delivery of mild electric shock relative to sets of matched cues that signal the absence of shock (no-threat cues). In the Threat Probability Task, fear is measured via startle potentiation to high probability (100% cue-contingent shock; certain) threat cues whereas anxiety is measured via startle potentiation to low probability (20% cue-contingent shock; uncertain) threat cues. Measurement of startle potentiation during the Threat Probability Task provides an objective and easily implemented alternative to assessment of negative affect via self-report or other methods (e.g., neuroimaging) that may be inappropriate or impractical for some researchers. Startle potentiation has been studied rigorously in both animals (e.g., rodents, non-human primates) and humans which facilitates animal-to-human translational research. Startle potentiation during certain and uncertain threat provides an objective measure of negative affective and distinct emotional states (fear, anxiety) to use in research on psychopathology, substance use/abuse and broadly in affective science. As such, it has been used extensively by clinical scientists interested in psychopathology etiology and by affective scientists interested in individual differences in emotion.

Potentiation of the startle reflex is measured via electromyography of the orbicularis oculi muscle during low (uncertain) and high (certain) probability electric shock threat in the Threat Probability Task. This provides an objective measure of distinct negative emotional states (fear/anxiety) for research on psychopathology, substance use/abuse, and broad affective science.

Fear of certain threat and anxiety about uncertain threat are distinct emotions with unique behavioral, cognitive-attentional, and neuroanatomical ...

The Attentional Set Shifting Task: A Measure of Cognitive Flexibility in Mice

Cognitive impairment, particularly involving dysfunction of circuitry within the prefrontal cortex (PFC), represents a core feature of many neuropsychiatric and neurodevelopmental disorders, including depression, post-traumatic stress disorder, schizophrenia and autism spectrum disorder. Deficits in cognitive function also represent the most difficult symptom domain&#160;to successfully treat, as serotonin reuptake inhibitors and tricyclic antidepressants have only modest effects. Functional neuroimaging studies and postmortem analysis of human brain tissue implicate the PFC as being a primary region of dysregulation in patients with these disorders. However, preclinical behavioral assays used to assess these deficits in mouse models which can be readily manipulated genetically and could provide the basis for studies of new treatment avenues have been underutilized. Here we describe the adaptation of a behavioral assay, the attentional set shifting task (AST), to be performed in mice to assess prefrontal cortex mediated cognitive deficits. The neural circuits underlying behavior during the AST are highly conserved across humans, nonhuman primates and rodents, providing excellent face, construct and predictive validity.

The goal of this protocol is to perform a behavioral assay such as the attentional set shifting task (AST) to assess prefrontal cortex-mediated cognitive flexibility in mice.

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Community-based Adapted Tango Dancing for Individuals with Parkinson's Disease and Older Adults

Adapted tango dancing improves mobility and balance in older adults and additional populations with balance impairments. It is composed of very simple step elements. Adapted tango involves movement initiation and cessation, multi-directional perturbations, varied speeds and rhythms. Focus on foot placement, whole body coordination, and attention to partner, path of movement, and aesthetics likely underlie adapted tango&#8217;s demonstrated efficacy for improving mobility and balance. In this paper, we describe the methodology to disseminate the adapted tango teaching methods to dance instructor trainees and to implement the adapted tango by the trainees in the community for older adults and individuals with Parkinson&#8217;s Disease (PD). Efficacy in improving mobility (measured with the Timed Up and Go, Tandem stance, Berg Balance Scale, Gait Speed and 30 sec chair stand), safety and fidelity of the program is maximized through targeted instructor and volunteer training and a structured detailed syllabus outlining class practices and progression.

Community-based Adapted Tango Dancing for Individuals with Parkinson&#039;s Disease and Older Adults

Adapted tango has demonstrated efficacy for improving mobility and balance. We describe the dissemination of adapted tango teaching methods to dance instructor trainees and the implementation of adapted tango by the trainees in the community for improving mobility and balance in older adults and individuals with Parkinson&#8217;s disease.

Adapted tango dancing improves mobility and balance in older adults and additional populations with balance impairments. It is composed of very simple ...

A Dual Task Procedure Combined with Rapid Serial Visual Presentation to Test Attentional Blink for Nontargets

When viewers search for targets in a rapid serial visual presentation (RSVP) stream, if two targets are presented within about 500 msec of each other, the first target may be easy to spot but the second is likely to be missed. This phenomenon of attentional blink (AB) has been widely studied to probe the temporal capacity of attention for detecting visual targets. However, with the typical procedure of AB experiments, it is not possible to examine how the processing of non-target items in RSVP may be affected by attention. This paper describes a novel dual task procedure combined with RSVP to test effects of AB for nontargets at varied stimulus onset asynchronies (SOAs). In an exemplar experiment, a target category was first displayed, followed by a sequence of 8 nouns. If one of the nouns belonged to the target category, participants would respond &#8216;yes&#8217; at the end of the sequence, otherwise participants would respond &#8216;no&#8217;. Two 2-alternative forced choice memory tasks followed the response to determine if participants remembered the words immediately before or after the target, as well as a random word from another part of the sequence. In a second exemplar experiment, the same design was used, except that 1) the memory task was counterbalanced into two groups with SOAs of either 120 or 240 msec and 2) three memory tasks followed the sequence and tested remembrance for nontarget nouns in the sequence that could be anywhere from 3 items prior the target noun position to 3 items following the target noun position. Representative results from a previously published study demonstrate that our procedure can be used to examine divergent effects of attention that not only enhance targets but also suppress nontargets. Here we show results from a representative participant that replicated the previous finding.&#160;

This paper describes a novel dual task procedure combined with Rapid Serial Visual Presentation to look at attentional blink at varied stimulus onset asynchronies. This experimental procedure differed from others in that it tested attentional blink for nontargets.

When viewers search for targets in a rapid serial visual presentation (RSVP) stream, if two targets are presented within about 500 msec of each other, the ...

The Modified Hole Board - Measuring Behavior, Cognition and Social Interaction in Mice and Rats

This protocol describes the modified hole board (mHB), which combines features from a traditional hole board and open field and is designed to measure multiple dimensions of unconditioned behavior in small laboratory mammals (e.g., mice, rats, tree shrews and small primates). This paradigm is a valuable alternative for the use of a behavioral test battery, since a broad behavioral spectrum of an animal&#8217;s behavioral profile can be investigated in one single test.
The apparatus consists of a box, representing the &#8216;protected&#8217; area, separated from a group compartment. A board, on which small cylinders are staggered in three lines, is placed in the center of the box, representing the &#8216;unprotected&#8217; area of the set-up. The cognitive abilities of the animals can be measured by baiting some cylinders on the board and measuring the working and reference memory. Other unconditioned behavior, such as activity-related-, anxiety-related- and social behavior, can be observed using this paradigm. Behavioral flexibility and the ability to habituate to a novel environment can additionally be observed by subjecting the animals to multiple trials in the mHB, revealing insight into the animals&#8217; adaptive capacities.
Due to testing order effects in a behavioral test battery, na&#239;ve animals should be used for each individual experiment. By testing multiple behavioral dimensions in a single paradigm and thereby circumventing this issue, the number of experimental animals used is reduced. Furthermore, by avoiding social isolation during testing and without the need to food deprive the animals, the mHB represents a behavioral test system, inducing if any, very low amount of stress.

This protocol describes the modified hole board, which is a behavioral test set-up that comprises the characteristics of an open field and a traditional hole board. This set-up enables the differential analysis of unconditioned behavior of small laboratory mammals as well as the analysis of cognitive abilities.

This protocol describes the modified hole board (mHB), which combines features from a traditional hole board and open field and is designed to measure ...

A Treatment Package without Escape Extinction to Address Food Selectivity

Feeding difficulties and feeding disorders are a commonly occurring problem for young children, particularly children with developmental delays including autism. Behavior analytic interventions for the treatment of feeding difficulties oftentimes include escape extinction as a primary component of treatment. The use of escape extinction, while effective, may be problematic as it is also associated with the emergence of challenging behavior (e.g., extinction burst). Such challenging behavior may be an acceptable side effect in treatment cases where feeding problems are severe and chronic (e.g., failure to thrive). However, in more acute cases (e.g., selective eating), the negative side effect may be unwarranted and undesired. More recent research on the behavioral treatment of food selectivity has begun to evaluate treatments for feeding difficulties that do not include escape extinction (e.g., demand fading, behavioral momentum), with some success. However, research to date reveals individual differences in responsiveness to such treatments and no clear preferable treatment has emerged. This manuscript describes a multi-component treatment package that includes shaping, sequential presentation and simultaneous presentation, for the treatment of food selectivity in four young children with developmental delays. This treatment package extends the literature on the behavioral treatment for food selectivity and offers a multi-component treatment protocol that may be clinically applicable across a range of treatment scenarios and settings.

Feeding difficulties are a common problem for children with developmental disorders, including autism, and behavioral interventions often include escape extinction. Recent research has begun to evaluate treatments that do not include escape extinction. This manuscript describes a multicomponent treatment package that does not use escape extinction to treat feeding difficulties.

Feeding difficulties and feeding disorders are a commonly occurring problem for young children, particularly children with developmental delays including ...

Psychophysical Tracking Method to Measure Taste Preferences in Children and Adults

The Monell two-series, forced-choice, paired-comparison tracking method provides a reliable measure of sweet taste preferences from childhood to adulthood. The method, which is identical for children, adolescents, and adults, is of short duration (&#60; 15 min), does not rely on sustained attention or place demands on memory (which would yield spurious age differences), and minimizes the impact of language development, making this method amenable to the cognitive limitations of pediatric populations. In this whole-mouth tasting method, subjects are asked to taste (without swallowing) pairs of solutions of different sucrose concentrations and to point to the solution they prefer. Each subsequent pair contains the participant&#39;s preceding preferred concentration and an adjacent stimulus concentration. The procedure continues until the subject chooses either a given concentration of sucrose when paired with both a higher and a lower concentration, or the highest or lowest concentration two consecutive times. Subjects are prevented from reaching response criteria on the basis of first or second position bias by the two-series design of the method, which counterbalances the order of solution presentation within each pair between the series (the weaker concentration is presented first in Series 1, second in Series 2). The geometric mean of the two sucrose concentrations chosen in Series 1 and 2 is an estimate of the participant&#39;s most preferred level of sucrose. Sucrose preference as determined with this laboratory-based measure has been shown to be associated with preference for sugars in foods and beverages and with taste receptor genotype, family history of alcoholism, and race/ethnicity, as well as depressive symptomatology among pediatric populations. The method has real-world relevance and has been applied to determine most preferred level of other tastes (e.g., salt), making it a valuable psychophysical tool.

Sweet taste has powerful hedonic appeal among people of all ages, particularly children. Described herein is a reliable and valid method that can be used to determine the level of sweetness most preferred, making it a valuable psychophysical tool for scientists.

The Monell two-series, forced-choice, paired-comparison tracking method provides a reliable measure of sweet taste preferences from childhood to ...

Assessing Working Memory in Children: The Comprehensive Assessment Battery for Children &#8211; Working Memory (CABC-WM)

The Comprehensive Assessment Battery for Children - Working Memory (CABC-WM) is a computer-based battery designed to assess different components of working memory in young school-age children. Working memory deficits have been identified in children with language-based learning disabilities, including dyslexia1,2 and language impairment3,4, but it is not clear whether these children exhibit deficits in subcomponents of working memory, such as visuospatial or phonological working memory. The CABC-WM is administered on a desktop computer with a touchscreen interface and was specifically developed to be engaging and motivating for children. Although the long-term goal of the CABC-WM is to provide individualized working memory profiles in children, the present study focuses on the initial success and utility of the CABC-WM for measuring central executive, visuospatial, phonological loop, and binding constructs in children with typical development. Immediate next steps are to administer the CABC-WM to children with specific language impairment, dyslexia, and comorbid specific language impairment and dyslexia.

Assessing Working Memory in Children: The Comprehensive Assessment Battery for Children &amp;#8211; Working Memory (CABC-WM)

Working memory predicts a significant amount of variance for a variety of cognitive tasks, including speaking, reading, and writing. However, few tools are available to assess working memory in children. We present an innovative, computer-based battery that comprehensively assesses different components of working memory in school-age children.

The Comprehensive Assessment Battery for Children - Working Memory (CABC-WM) is a computer-based battery designed to assess different components of ...