Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
16.5K Views
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11:26 min
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January 18th, 2015
DOI :
January 18th, 2015
•Jerome D. Robin1, Woody E. Wright1, Yaqun Zou2, Stacy A. Cossette3, Michael W. Lawlor3, Emanuela Gussoni4
1Department of Cell Biology, UT Southwestern Medical Center, 2National Institute of Neurological Disorders and Stroke, National Institute of Health, 3Division of Pediatric Pathology, Department of Pathology and Laboratory Medicine, Medical College of Wisconsin, 4Division of Genetics and Genomics, Boston Children's Hospital
Chapters in this video
0:05
Title
1:27
Myogenic Cell Isolation
1:27
Myogenic Cell Isolation
4:33
Preparing the Virus Aliquots
6:47
Immortalizing the Myogenic Cells
8:20
Clonal Selection of Immortalized Myogenic Cells
9:30
Results: Characterizing Immortalized Myogenic Cells
10:35
Conclusion
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