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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

17.8K Views

10:26 min

September 10th, 2015

DOI :

10.3791/52985-v

September 10th, 2015


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TRAP

Chapters in this video

0:05

Title

2:09

Amplification of TRAP Line

4:29

Lysate Preparation

6:10

Pre-absorption

7:03

Immunopurification

8:35

Results: Quality and Specificity of TRAP-Isolated RNA from Slouch-GAL4>UASRpL10A-EGFP Embryos

9:56

Conclusion

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