Digestion and Vascular Smooth Muscle Cell (VSMC) Isolation
6:08
VSMC Culture
7:10
Results: Representative Cultured Coronary VSMC
7:58
Conclusion
Transcribir
The overall goal of this procedure is to isolate vascular smooth muscle cells from the murine coronary circulation for their culture and use in standard cell culture assays. This method can help answer key questions in the cardiovascular field suc
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The purpose of this protocol is to demonstrate the isolation and culture techniques of murine primary vascular smooth muscle cells (VSMCs) from the coronary circulation. Once VSMCs have been isolated, they can be used for many standard culture techniques.