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Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

DOI :

10.3791/58193-v

12:21 min

January 7th, 2019

January 7th, 2019

12,271 Views

1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.

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Recombinant Avian Herpesvirus Vectors

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