In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
7.8K Views
•
05:45 min
•
January 9th, 2020
DOI :
January 9th, 2020
•1The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University of the Negev, 2Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Human Oncology & Pathogenesis Program (HOPP), Memorial Sloan Kettering Cancer Center, 4Institute of Pathology, Barzilai University Medical Center
Chapters in this video
0:04
Title
0:41
Primary Cell Isolation and Culture
2:35
Adeno-Associated Virus (AAV) Primary Cell Transduction
3:37
Results: Representative In Vitro Transformation of Primary Mouse Embryonic Fibroblast (MEF) and Tongue Epithelial Cells Using the AAV-Cas9 System
5:10
Conclusion
Related Videos
08:59
A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
10.5K Views
07:43
Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
9.6K Views
06:01
A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
9.4K Views
07:39
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
9.2K Views
06:11
Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
9.7K Views
08:32
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
9.5K Views
11:06
Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System
10.3K Views
10:33
Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
27.1K Views
08:42
Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
9.7K Views
08:36
In Vitro Assay to Study Tumor-macrophage Interaction
7.4K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved