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How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

DOI :

10.3791/58666-v

7:22 min

February 11th, 2019

February 11th, 2019

26,445 Views

1Department of Biosciences, Durham University, 2Department of Chemistry, Durham University

A protocol is presented to rapidly test the thermal stability of proteins in a variety of conditions through thermal shift assays and nano differential scanning fluorimetry. Buffer systems, salts and additives, together comprising three unique stability screens, are assayed with proteins to identify suitable buffers for functional and structural studies.

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Stabilize Protein

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