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Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

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11:08 min

January 7th, 2019

January 7th, 2019

20,306 Views

0:04

Title

0:42

Setup of Bubble Column Photobioreactors

2:13

Cultivation of Microalgae in Bubble Column Photobioreactors

4:03

Harvest and Freeze Drying of Microalgal Biomass

5:28

Lipid Extraction Using a Modified Folch Method

7:07

Neutral Lipid Assay Using a Microplate Method (Adapted from Higgins et al. 201422)

9:01

Results: Auxenochlorella protothecoides Growth Results and Chlorella sorokiniana Neutral Lipid Data

10:35

Conclusion

Transcript

This protocol provides instructions for the construction and use of a Bubble Column Photobioreactor system to grow microalgae, and should be of interest to anyone studying algel biofuels, wastewater treatment, or algel biology. We have found the s

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Here, we present a protocol to construct lab-scale bubble column photobioreactors and use them to culture microalgae. It also provides a method for the determination of the culture growth rate and neutral lipid content.

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