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A High-throughput Shigella-specific Bactericidal Assay

DOI :

10.3791/59164-v

6:11 min

February 27th, 2019

February 27th, 2019

10,892 Views

1Department of Enteric Infections, Bacterial Diseases Branch, Walter Reed Army Institute of Research, 2University of Alabama at Birmingham

Here we present a protocol to measure Shigellacidal activity of antibodies in serum. Serum is mixed with bacteria and exogenous complement, incubated, and the reaction mixture is plated on agar plates. Viable bacteria form colonies which are counted, using an automated colony enumerator, and used to determine the bactericidal titer.

Tags

High throughput

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