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Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis

19.5K Views

10:31 min

May 10th, 2019

DOI :

10.3791/59681-v

May 10th, 2019


Transcript

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ECLIP

Chapters in this video

0:04

Title

1:14

Tissue Harvesting and UV Crosslinking

2:37

Beads Preparation

3:20

Tissue Lysis and Partial RNA Digestion

4:30

Immunoprecipitation

5:09

RNA Adapter Ligation to RNA 3’ Ends

6:48

SDS-PAGE and Membrane Transfer

8:24

Results: Analysis of the eCLIP Method

10:08

Conclusion

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