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Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route Video (Video) | JoVE

DOI :

10.3791/63574-v

5:35 min

May 31st, 2022

May 31st, 2022

2,329 Views

1Department of Neuropharmacology, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 2GLIA Center, University of Yamanashi

The protocol presented here allows the transplantation of induced pluripotent stem cell-derived human microglia (iPSMG) into the brain via a transnasal route in immunocompetent mice. The method for the preparation and transnasal transplantation of cells and the administration of cytokine mixture for the maintenance of iPSMG is shown.

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Transplantation

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