Results: Fibroblasts in Injured and Uninjured Mouse Hearts
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Conclusion
Transcript
This protocol allows us to isolate both quiescent and injury-induced activated fibroblast populations with significant purity. The main advantage of this technique is its flexibility for improvisation. For flow, as well as bead-based isolation, on
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Obtaining a pure population of fibroblasts is crucial to studying their role in wound repair and fibrosis. Described here is a detailed method to isolate fibroblasts and myofibroblasts from uninjured and injured mouse hearts followed by characterization of their purity and functionality by immunofluorescence, RTPCR, fluorescence-assisted cell sorting, and collagen gel contraction.