An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
5.7K Views
•
13:46 min
•
July 11th, 2020
DOI :
July 11th, 2020
•Nicolás Stuardo1,2, Guillermo Moya-Alvarado1,3, Carolina Ramírez1,3, Giampietro Schiavo4, Francisca C. Bronfman1,3
1Department of Physiology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, 2Department of Molecular and Cellular Biology, Faculty of Biological Sciences, Center for Aging and Regeneration (CARE UC), Pontificia Universidad Católica de Chile, 3Institute of Biomedical Sciences. Faculty of Medicine and Faculty of Life Sciences, Universidad Andrés Bello, 4Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and UK Dementia Research Institute at UCL, University College London Campus
Chapters in this video
0:00
Introdcution
0:48
Transfection of HEK293 cells
1:49
Media Collection and Storage
3:02
Media Concentration and Purification
4:58
In vitro Mono-biotinylation Reaction
6:01
Biotinylation Analysis
6:56
Visualization of Retrograde Axonal Transport of BDNF-QD in Live Neurons
7:58
Represetative Results
13:17
Conclusion
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