Results: Cellular Target Engagement of Allosteric SHP2 Inhibitors with SHP2-WT and SHP2-E76K Proteins
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Transcript
The tyrosine phosphatase SHP2, is a key mediator of cellular signaling, promoting cell survival and proliferation. My team is focused on finding small molecule SHP2 inhibitors for the treatment of cancer. Our technique provides a rapid and powerfu
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The ability to assess target engagement by candidate inhibitors in intact cells is crucial for drug discovery. This protocol describes a 384 well format cellular thermal shift assay that reliably detects cellular target engagement of inhibitors targeting either wild-type SHP2 or its oncogenic variants.