A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development

3.7K Views

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08:50 min

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June 24th, 2020

DOI :

10.3791/61464-v

June 24th, 2020

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Brian A. Juber¹, Timothy G. Elgin¹, Erin M. Fricke², Huyiu Gong¹, Jeffrey Reese³, Steven J. McElroy¹^,⁴

¹Division of Neonatology, Stead Family Department of Pediatrics, University of Iowa, ²Division of Maternal Fetal Medicine, Department of Obstetrics & Gynecology, University of Iowa, ³Division of Neonatology, Department of Pediatrics, Vanderbilt University, ⁴Department of Microbiology & Immunology, University of Iowa

Summary

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Murine Model

Fetal Exposure

Maternal Inflammation

Chorioamnionitis

Newborn Intestinal Development

Necrotizing Enterocolitis

FEMI Protocol

LPS Injection

Gestation Day E15

Negative Pressure Injection

Vaginal Delivery E20

Postnatal Day 14

RNA Quantification

Paraffin embedded Tissue

Chapters in this video

0:04

Introduction

0:52

Establishment of FEMI in Pregnant Mice

2:06

Delivery and Care of Offspring and Intestinal Harvesting

3:03

Intestinal Injury Scoring

4:11

Quantification of Paneth and Goblet Cells

6:35

Results: Intestinal Injury of Newborn Mice Induced by FEMI

8:22

Conclusion

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