Pellet Preparation and Soluble Biomaterial Extracts Acquisition
2:11
Morphology Evaluation
3:28
Cell Function Assessment
5:54
Mineralized Formation Assessment
6:50
Results: Cell Viability, Morphology, and Protein Expression in Response to Biomaterial Exposure
8:30
Conclusion
Transcript
With the explosion of new materials for medical applications, the initial screening should be made systematically, so this protocol can be a fundamental tool for researchers aiming to develop and characterize novel biomaterials. The methodology al
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This methodology aims to evaluate biomaterial cytotoxicity through the preparation of soluble extracts, using viability assays and phenotypic analysis, including flow cytometry, RT-PCR, immunocytochemistry, and other cellular and molecular biology techniques.