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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DOI :

10.3791/62073-v

December 29th, 2021

December 29th, 2021

3,477 Views

1Institute of Biomedical Engineering, University of Toronto

We describe the engineering of a novel DNA-tethered T7 RNA polymerase to regulate in vitro transcription reactions. We discuss the steps for protein synthesis and characterization, validate proof-of-concept transcriptional regulation, and discuss its applications in molecular computing, diagnostics, and molecular information processing.

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