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Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

5.3K Views

06:43 min

May 26th, 2021

DOI :

10.3791/62468-v

May 26th, 2021


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Ussing Chamber Technique

Chapters in this video

0:04

Introduction

0:55

Stripping the Muscle Layer and Preparing the Intestinal Sheet

2:12

Mounting Intestinal Preparations in Ussing Chambers

3:05

Measurement of Dilution Potential (Open Circuit Conditions)

4:07

Transepithelial Electrical Conductance and Baseline Isc (Short-Circuit Conditions)

4:55

Results: Transepithelial Electrical Conductance and Dilution Potential in Cldn15-/- Mice

6:12

Conclusion

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