Stripping the Muscle Layer and Preparing the Intestinal Sheet
2:12
Mounting Intestinal Preparations in Ussing Chambers
3:05
Measurement of Dilution Potential (Open Circuit Conditions)
4:07
Transepithelial Electrical Conductance and Baseline Isc (Short-Circuit Conditions)
4:55
Results: Transepithelial Electrical Conductance and Dilution Potential in Cldn15-/- Mice
6:12
Conclusion
Transcript
This protocol is used to study the transport qualities of tight junctions. Using dilution potentials, you can measure the permselectivity and get an understanding of tight junctions in a tissue. This technique is specific and uses native tissues f
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Intestinal epithelium confers not only nutrient absorption but protection against noxious substances. The apical-most epithelial intercellular junction, i.e., the tight junction, regulates paracellular solute and ion permeability. Here, a protocol for the preparation of mucosal sheets and assessment of the ion selectivity of tight junctions using Ussing chamber technique is described.