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Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

DOI :

10.3791/62468-v

May 26th, 2021

May 26th, 2021

4,028 Views

1Laboratory of Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka

Intestinal epithelium confers not only nutrient absorption but protection against noxious substances. The apical-most epithelial intercellular junction, i.e., the tight junction, regulates paracellular solute and ion permeability. Here, a protocol for the preparation of mucosal sheets and assessment of the ion selectivity of tight junctions using Ussing chamber technique is described.

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