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Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement

DOI :

10.3791/62511-v

August 26th, 2021

August 26th, 2021

2,514 Views

1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

It is now acknowledged that the three-dimensional environment of cells can play an important role in their behavior, maturation and/or differentiation. This protocol describes a three-dimensional cell culture model designed to study the impact of physical containment and mechanical constraints on megakaryocytes.

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