Results: Megakaryocyte Characteristics and Proplatelet Quantification According to Culture Conditions
7:08
Conclusion
Transcrição
This protocol allows for in vitro evaluation of the impact of confinement and medium stiffness to which native megakaryocytes are exposed to in the bone marrow on their behavior and maturation. The main advantage of this technique is a simplified
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It is now acknowledged that the three-dimensional environment of cells can play an important role in their behavior, maturation and/or differentiation. This protocol describes a three-dimensional cell culture model designed to study the impact of physical containment and mechanical constraints on megakaryocytes.