All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

2.9K Views

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09:55 min

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December 20th, 2021

DOI :

10.3791/62934-v

December 20th, 2021

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Qin Luo*¹, Miao Huang*², Chenyu Liang², Justin Zhang³, Gaoming Lin¹, Sydney Yu¹, Mai Tanaka⁴^,⁵, Sharon Lepler⁴^,⁵, Juan Guan⁵^,⁶^,⁷, Dietmar Siemann⁴^,⁵, Xin Tang²^,⁵

¹Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering, University of Florida, ²Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, ³Department of Electrical and Computer Engineering, University of California, ⁴Department of Radiation Oncology, College of Medicine, University of Florida, ⁵UF Health Cancer Center, University of Florida, ⁶Department of Physics, College of Liberal Arts and Sciences, University of Florida, ⁷Department of Anatomy and Cell Biology, College of Medicine, University of Florida

Summary

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Mechanobiology

Yes associated Protein

Optical Electrophysiology

Super resolution Imaging

Live cell Imaging

High throughput

Fluorescence Microscopy

Automatic Image Acquisition

Environmental Chamber

Chapters in this video

0:04

Introduction

0:53

Cell Imaging

7:21

Results: All-Optical Mechanobiology Interrogation of Yes-Associated Protein in Human Cancer and Normal Cells