Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

1.6K Views

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12:53 min

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March 16th, 2022

DOI :

10.3791/63028-v

March 16th, 2022

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Sean F. Gilmore*¹, Wei He*¹, Angela C. Evans¹, Delia F. Tifrea², Sukumar Pal², Brent Segelke¹, Sandra K. G. Peters¹, B. Dillon Vannest¹, Nicholas O. Fischer¹, Amy Rasley¹, Luis M. de la Maza², Matthew A. Coleman¹^,³

¹Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, ²Department of Pathology and Laboratory Medicine, University of California, ³School of Medicine, Radiation Oncology, University of California Davis

Summary

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Cell Free Production

Recombinant Protein

Major Outer Membrane Protein

Reconstitution Buffer

Amino Acid Mixture

GFP Control

Chapters in this video

0:04

Introduction

1:00

Cell‐Free Production of MOMP‐tNLPs for Subunit Vaccine Formulations

4:21

MOMP‐tNLP Purification

7:19

Western and Dot Blots and Storage

10:14

Results: SDS‐PAGE Analysis

11:46

Conclusion

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