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Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

4.4K Views

08:21 min

May 16th, 2022

May 16th, 2022

4,354 Views

0:04

Introduction

0:46

Soil Sampling and Dilutions

2:35

Growth Tests on Recalcitrant Substances

5:00

Qualitative Enzymatic Tests

6:26

Results: Isolation and Screening of Fungal Strains for the Degradation of Recalcitrant Materials

7:52

Conclusion

Transcript

This protocol is significant because it isolates fungal strains with high degradative potential directly from soil samples to be considered as candidates for bioremediation purposes. This protocol is a low-cost screening to find fungi with bioreme

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Here, we present a protocol for screening soil biodiversity to look for fungal strains involved in the degradation of recalcitrant materials. First, fungal strains able to grow on humic acids or lignocellulose are isolated. Their activity is then tested both in enzymatic assays and on pollutants such as hydrocarbons and plastics.

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