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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

DOI :

10.3791/63940-v

8:45 min

April 21st, 2022

April 21st, 2022

1,861 Views

1Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, 2Department of Chemistry, University of Illinois Urbana-Champaign

Post-transcriptional modifications of RNA represent an understudied layer of translation regulation that has recently been linked to central nervous system plasticity. Here, sample preparation and liquid chromatography-tandem mass spectrometry approach is described for simultaneous characterization of numerous RNA modifications in single neurons.

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RNA Modifications

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