Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia
2.0K Views
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09:43 min
•
June 9th, 2023
DOI :
June 9th, 2023
•Gisele O. L. Rodrigues1, WenQing Li1, Sarah D. Cramer1,2,3, Hila Y. Winer1, Tu Chun Hsu1,2,3, Timothy Gower4, Julie A. Hixon1, Scott K. Durum1
1Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute, National Institutes of Health, 2Comparative Biomedical Scientist Training Program, NIH, 3Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory, Michigan State University, 4NCI-Frederick Laboratory Animal Sciences Program
Chapters in this video
0:04
Introduction
0:47
Seeding and Transfecting the Retrovirus-Producer Cells
2:31
Thymocyte Preparation and Depletion of CD4+ and CD8+ Cells
4:53
Harvesting Retrovirus and Transducing the Thymocytes
7:13
Results: Thymocyte Depletion and Thymocyte Retroviral Transduction Efficiency
8:51
Conclusion
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