Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

1.9K Views

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09:43 min

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June 9th, 2023

DOI :

10.3791/64271-v

June 9th, 2023

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Gisele O. L. Rodrigues¹, WenQing Li¹, Sarah D. Cramer¹^,²^,³, Hila Y. Winer¹, Tu Chun Hsu¹^,²^,³, Timothy Gower⁴, Julie A. Hixon¹, Scott K. Durum¹

¹Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute, National Institutes of Health, ²Comparative Biomedical Scientist Training Program, NIH, ³Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory, Michigan State University, ⁴NCI-Frederick Laboratory Animal Sciences Program

Summary

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T cell Leukemia

Thymocytes

Retroviral Vectors

OP9 DL4 Stromal Cells

Gene Transduction

Post doctoral Fellow

Genetic Modifications

Cell Culture

Transfection Protocol

Lipofection Mixtures

Retrovirus Producer Cells

Transfection Reagent

Thymocyte Suspension

Cancer Research

Chapters in this video

0:04

Introduction

0:47

Seeding and Transfecting the Retrovirus-Producer Cells

2:31

Thymocyte Preparation and Depletion of CD4⁺ and CD8⁺ Cells

4:53

Harvesting Retrovirus and Transducing the Thymocytes

7:13

Results: Thymocyte Depletion and Thymocyte Retroviral Transduction Efficiency

8:51

Conclusion

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