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In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer

DOI :

10.3791/50322-v

February 22nd, 2013

February 22nd, 2013

14,363 Views

1Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine

The endothelial glycocalyx/endothelial surface layer is ideally studied using intravital microscopy. Intravital microscopy is technically challenging in a moving organ such as the lung. We demonstrate how simultaneous brightfield and fluorescent microscopy may be used to estimate endothelial surface layer thickness in a freely-moving in vivo mouse lung.

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