In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening

1.4K Views

08:16 min

October 6th, 2022

DOI :

10.3791/64392-v

October 6th, 2022


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In Vitro Cleavage Assays

Chapters in this video

0:04

Introduction

1:15

Small‐Scale Recombinant Caspases Extraction

2:34

Small‐Scale His‐Tagged Caspases Purification

4:09

In Vitro Cleavage Assay with Substrates Generated with RRL

5:29

Results: In Vitro Cleavage Assays to Express and Purify Recombinant Drosophila Caspases

7:33

Conclusion

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