A Silicosis Mouse Model Established by Repeated Inhalation of Crystalline Silica Dust
2.1K Views
•
10:45 min
•
January 6th, 2023
DOI :
January 6th, 2023
•Hangbing Cao*1,2,3,4, Bing Li*1,2,3,4, Haoming Chen1,3,4, Yehong Zhao1,4, Yuanjie Zou1,4, Yang Liu1,4, Min Mu1,2,3,4, Xinrong Tao1,2,3,4
1Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, 2Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes, Anhui University of Science and Technology, 3Anhui Province Engineering Laboratory of Occupational Health and Safety, 4School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology
Chapters in this video
0:04
Introduction
1:07
Preparing the Crystalline Silica Dust (CSD) Suspension
1:52
Administering the Nasal Drips to the Mouse
2:24
Performing Hematoxylin and Eosin (HE) Staining
3:23
Performing Masson Staining and Sirius Red Staining
4:27
Performing Immunohistochemistry
6:10
Performing Western Blotting Analysis
8:02
Results: Investigating the Development of Silicosis in Mice After Repeated CSD Inhalation
9:41
Conclusion
Related Videos
09:11
A Mouse Model of in Utero Transplantation
19.3K Views
08:46
An Improved Method for Rapid Intubation of the Trachea in Mice
25.1K Views
06:52
Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin
17.1K Views
08:17
Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure
10.8K Views
05:03
Establishing a Silicosis Rat Model via Exposure of Whole-Body to Respirable Silica
1.2K Views
06:12
Using Nicotine in a Silica-Exposed Mouse Model to Promote Lung Epithelial-Mesenchymal Transition
672 Views
09:29
An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing
11.3K Views
02:46
A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization
3.4K Views
05:43
Establishment of a Mouse Model with Cough Hypersensitivity via Inhalation of Citric Acid
377 Views
01:20
Establishing a Mouse Model of Pleural Disseminated Lung Cancer
261 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved