A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

1.4K Views

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09:24 min

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March 10th, 2023

DOI :

10.3791/64927-v

March 10th, 2023

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Michael Landowski¹^,², Samuel Grindel¹, Ying Hao³, Sakae Ikeda¹^,², Catherine Bowes Rickman³^,⁴, Akihiro Ikeda¹^,²

¹Department of Medical Genetics, University of Wisconsin-Madison, ²McPherson Eye Research Institute, University of Wisconsin-Madison, ³Department of Ophthalmology, Duke University, ⁴Department of Cell Biology, Duke University

Summary

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Protocol

Retinal Pigmented Epithelium

Age related Macular Degeneration

Mouse Models

Quantitative Assessments

Microscopy Techniques

Chapters in this video

0:04

Introduction

1:04

Evaluation of Mouse Retinal Pigmented Epithelium (RPE) by Light Microscopy

5:29

Evaluation of Mouse RPE Through Confocal Microscopy

6:32

Results: Investigating RPE Pathologies in Mice

8:35

Conclusion

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