Preparation of Cells Expressing DiKillerRed or Self‐Labeling Proteins (SLPs) in the Peroxisome Lumen
2:52
Staining Peroxisomes with Dye‐Labeled SLP Ligands
3:39
ROS‐Stressing of Peroxisomes on a Laser‐Scanning Confocal Microscope
6:25
Results: Light‐Activated ROS Production and Stub1‐Mediated Pexophagy
7:50
Conclusion
Transcript
Peroxisomes carry out beta oxidation and are prone to ROS damage. This method allows to investigate how cells deal with ROS-stressed peroxisomes. This protocol is not only used to globally target all the peroxisomes within a cell population but ca
Sign in or start your free trial to access this content
This protocol provides instructions for triggering and monitoring Stub1-mediated pexophagy in live cells.