Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

974 Views

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11:53 min

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March 31st, 2023

DOI :

10.3791/65067-v

March 31st, 2023

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Jan van der Beek¹, Tineke Veenendaal¹, Cecilia de Heus¹, Suzanne van Dijk¹, Corlinda ten Brink¹, Nalan Liv¹, Judith Klumperman¹

¹Center for Molecular Medicine-Cell Biology, University Medical Center Utrecht, Utrecht University

Summary

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Ultrastructural Localization

Endogenous LC3

Correlative Light electron Microscopy

CLEM

Autophagy Proteins

Fluorescence Microscopy

Electron Microscopy

Cell Ultrastructure

Biological Research Protocol

Chapters in this video

0:05

Introduction

0:50

Sample Embedding

3:13

Sectioning

4:41

Labeling and Light Microscopy

9:18

Results: Correlative Light-EM of LC3 and LAMP1 in Starved HEPG2 Cells

11:16

Conclusion

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