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A GMP-Compliant Procedure for the Generation of Gene-Modified T cells

421 Views

06:47 min

October 6th, 2023

DOI :

10.3791/65097-v

October 6th, 2023


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GMP compliant Procedure

Chapters in this video

0:00

Introduction

0:38

Day 0: Peripheral Blood Mononuclear Cells (PBMC) Isolation and T Cell Activation

1:42

Day 3: Lentiviral T Cell Transduction

3:04

Day 4: Virus Removal and T Cell Expansion

3:44

Day 14: Cryopreservation

4:39

Transduction Efficiency-Flow Cytometry Staining (Day 14)

5:35

Results: Assessment of the Transduced Human T Cells

6:29

Conclusion

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