Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures

1.5K Views

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04:01 min

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June 23rd, 2023

DOI :

10.3791/65286-v

June 23rd, 2023

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Katie B. Grausam*¹, Joshua J. Breunig*¹^,²^,³^,⁴^,⁵

¹Board of Governor’s Regenerative Medicine Institute, Cedars-Sinai Medical Center, ²Center for Neural Sciences in Medicine, Cedars-Sinai Medical Center, ³Department of Biomedical Sciences, Cedars-Sinai Medical Center, ⁴Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, ⁵Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles

Summary

Explore More Videos

Brain Tumors

Glioblastoma

Patient Mutation Profiles

Immunocompetent Mouse Model

Tumor Microenvironment

Genetic Driver Mutations

Immune Cell Populations

Personalized Modeling

Somatic Mutagenesis

MADR Method

Chapters in this video

0:00

Introduction

1:53

Microinjection of DNA Plasmid Mix into the Mouse Brain Ventricle

2:36

Electroporation‐Based DNA Plasmids Delivery to Induce In Vivo Mutations in the Mouse

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