Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing
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05:59 min
•
May 3rd, 2024
DOI :
May 3rd, 2024
•Line O. Elingaard-Larsen1,2, Katie L. Whytock1, Adeline Divoux1, Meghan Hopf1, Erin E. Kershaw3, Jamie N. Justice4, Bret H. Goodpaster1, Nancy E. Lane5, Lauren M. Sparks1
1Translational Research Institute, AdventHealth, 2Steno Diabetes Center Copenhagen, 3Division of Endocrinology and Metabolism, University of Pittsburgh, 4Gerontology and Geriatric Medicine, Wake Forest University School of Medicine, 5Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology, University of California Davis Health
Chapters in this video
0:00
Introduction
2:05
Pulverization and Homogenization of Frozen Human Intermuscular Adipose Tissue Samples for Nuclei Isolation
3:41
Single‐Cell Nuclei Isolation from Homogenized Human Intermuscular Adipose Tissue Samples for RNA Sequencing
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