Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing
570 Views
•
05:59 min
•
May 3rd, 2024
DOI :
May 3rd, 2024
•Line O. Elingaard-Larsen1,2, Katie L. Whytock1, Adeline Divoux1, Meghan Hopf1, Erin E. Kershaw3, Jamie N. Justice4, Bret H. Goodpaster1, Nancy E. Lane5, Lauren M. Sparks1
1Translational Research Institute, AdventHealth, 2Steno Diabetes Center Copenhagen, 3Division of Endocrinology and Metabolism, University of Pittsburgh, 4Gerontology and Geriatric Medicine, Wake Forest University School of Medicine, 5Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology, University of California Davis Health
Chapters in this video
0:00
Introduction
2:05
Pulverization and Homogenization of Frozen Human Intermuscular Adipose Tissue Samples for Nuclei Isolation
3:41
Single‐Cell Nuclei Isolation from Homogenized Human Intermuscular Adipose Tissue Samples for RNA Sequencing
Related Videos
10:58
Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
22.1K Views
06:22
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
12.1K Views
07:03
Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
9.8K Views
Isolation and Processing of Murine White Adipocytes for Transcriptome and Epigenome Analyses
2.0K Views
06:38
Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
18.6K Views
07:23
Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
41.2K Views
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
19.6K Views
07:00
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
24.3K Views
09:09
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
5.5K Views
07:12
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
3.9K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved