Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

13.3K Views

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09:54 min

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April 18th, 2019

DOI :

10.3791/59297-v

April 18th, 2019

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Yena Kim¹^,², Ji Hyeon Ju¹^,²^,³

¹CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine, The Catholic University of Korea, ²Department of Biomedicine & Health Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ³Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea

Summary

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3D Skin Organoid

Cord Blood derived

Induced Pluripotent Stem Cells

Regenerative Medicine

Embryonic Body Formation

ROCK Inhibitor

Differentiation Medium

Dermatologic Research

Cell Culture Protocol

E8 Medium

Chapters in this video

0:04

Title

0:39

Embryonic Body (EB) Generation

2:39

Cord Blood Mononuclear Cell-derived-induced Pluripotent Stem Cell (CBMC-iPSC) Keratinocyte Differentiation

4:17

CBMC-iPSC Fibroblast Differentiation

5:50

3D Skin Organoid Generation

8:00

Results: Representative CBMC-iPSC-derived 3D Skin Organoid Formation

9:10

Conclusion

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