JoVE Journal

Immunology and Infection

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Immunofluorescence Imaging of Neutrophil Extracellular Traps in Human and Mouse Tissues

5.5K Views

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07:36 min

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August 18th, 2023

DOI :

10.3791/65272-v

August 18th, 2023

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Lavinia Schoenfeld¹, Birgit Appl¹, Laia Pagerols-Raluy¹, Annika Heuer³, Konrad Reinshagen¹, Michael Boettcher¹^,²

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, University of Hamburg, ²Department of Pediatric Surgery, University Medical Center Mannheim, University of Heidelberg, ³Division of Spine Surgery, Department of Trauma and Orthopedic Surgery, University Medical Center Hamburg-Eppendorf (UKE)

Summary

Neutrophil extracellular traps (NETs) are associated with various diseases, and immunofluorescence is often used for their visualization. However, there are various staining protocols, and, in many cases, only one type of tissue is examined. Here, we establish a generally applicable protocol for staining NETs in mouse and human tissue.

Explore More Videos

Immunofluorescence Imaging

Neutrophil Extracellular Traps

Antigen Retrieval

Antibody Selection

Autofluorescence

Standardized Protocols

Background Staining

Primary Antibody

Staining Methods

Sample Mounting

Therapeutic Intervention

Chapters in this video

0:00

Introduction

1:45

Autofluorescence Blocking and Antigen Retrieval in Tissue Samples

3:37

Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

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