Epon Post Embedding Correlative Light and Electron Microscopy
1.2K Views
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08:47 min
•
January 12th, 2024
DOI :
January 12th, 2024
•Shuyuan Wang*1, Haiyan Xiong*1,2, Qiyuan Chang*1,3, Xudong Zhuang*4,5,6, Yaochen Wu1,3, Xinrui Wang4,5,6, Congxian Wu1, Zhifei Fu1,7,8
1Public Technology Service Center, Fujian Medical University, 2The School of Pharmacy, Fujian Medical University, 3The School of Basic Medical Sciences, Fujian Medical University, 4College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 5Medical Research Center, Fujian Maternity and Child Health Hospital, 6NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, Fujian Maternity and Child Health Hospital, 7Institute of Neuroscience, Fujian Medical University, 8Fujian Key Laboratory of Molecular Neurology, Fujian Medical University
Chapters in this video
0:00
Introduction
0:44
Ultrathin Sectioning for Epon Post‐Embedding Correlative Light and Electron Microscopy (CLEM)
1:33
Creating the Navigation Map for CLEM and Preparation of the Ultrathin Sections for Electron Microscopy
4:07
Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post‐Embedding Correlative Light and Electron Microscopy
6:16
Registration of the Fluorescence Microscopy Image with the Electron Microscopy Image for Correlative Light and Electron Microscopy
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