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Developmental Biology

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Generating Kidney Organoids in Suspension from Induced Pluripotent Stem Cells

2.2K Views

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07:22 min

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September 1st, 2023

DOI :

10.3791/65698-v

September 1st, 2023

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Langping Gao*¹^,², Yue Wang*³, Gang Wang⁴, Hangdi Wu², Qingtao Yan¹, Jingjing Wang¹, Fei Liu¹, Haidong Fu¹, Wei Li⁵, Lidan Hu¹, Jianhua Mao¹^,²

¹Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, ²Zhejiang University School of Medicine, ³Hubei Normal University, ⁴National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, ⁵Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health

Summary

This protocol presents a comprehensive and efficient method for producing kidney organoids from induced pluripotent stem cells (iPSCs) using suspension culture conditions. The primary emphasis of this study lies in the determination of the initial cell density and the WNT agonist concentration, thereby benefiting investigators interested in kidney organoid research.

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Kidney Organoids

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Differentiation Ability

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Hereditary Kidney Disease

Protocol Optimization

Chapters in this video

0:00

Introduction

2:31

iPSC Differentiation into Posterior Primitive Streak and Nephrogenic Intermediate Mesoderm

4:50

Generating Kidney Organoids from Induced Pluripotent Stem Cell Suspension Culture

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